ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mohanad Jawad Kadhim , Shaimaa Obaid Hasson and Hayder Shkhair Al-Janabi
Department of Biotechnology, College of Biotechnology, Al-Qasim Green University, Iraq.
Article Number: 8219 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(2):861-869. https://doi.org/10.22207/JPAM.17.2.12
Received: 06 November 2022 | Accepted: 12 February 2023 | Published online: 17 April 2023
Issue online: June 2023
Abstract

Neisseria gonorrhoeae is a member of the Neisseriaceae family. They are non-spore-forming, gram-negative, encapsulated, non-motile, non-acidic, and bean-shaped bacteria. This study included 40 men that presented chronic and recurrent infection with N. gonorrhoeae virus. The initial infection of this virus was detected using a rapid bacterial antigen immunoassay and confirmed using enzyme-linked immunosorbent assay (ELISA ). The mean age of the study group was 36.71±12 (mean±) years.  Men between the age of 20-49 years were the most affected by N. gonorrhoeae infection, and displayed a significantly lower average sperm count than the healthy individuals upon semen examination. The percentage of sperms with slow motility, total number of dead sperms, and abnormal- shaped sperms were some significant phenotypes observed in the infected individuals as compared to that in the healthy controls. In this study, we found that the bacterium, N. gonorrhoeae could cause erosion of the mitochondrial DNA of sperms in the semen of the infected individuals. In addition, using the gap-PCR technique, it became evident that the infected individuals portraying altered sperm characteristics as mentioned above, showed increased number of common deletion  (4, 977 base pairs) in the sperm mitochondrial (mt)-DNA. Hence, our results imply that N. gonorrhoeae infection can lead to a common deletion of  4,977 bp in sperm mt–DNA, which can in turn cause male sterility.

Keywords

mt-DNA, Men Sperm, N. gonorrhea

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.