Maryam Hosseini1, Fateme Babaha1, Mushtaq T. Sh. Al-Rubaye1*,
Javad Fakhari1 and Mastafa H. J. Al-Musawi2

1Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
2College of Pharmacy, University of Al-Mustansriyah, Baghdad, Iraq.

Abstract

Salt lakes represent an extreme habitat for prokaryotes which tolerate significant concentration of NaCl. Many studies on screening and characterization of these microorganisms have been done, however, very little reported about urease producer halophilic bacteria. The present study aimed to isolate urease positivebacteria from Bahr Al-Milh Salt Lake, Karbala, Iraq.A total of 161 gram positive and 57 gram negative bacteria were found which one third of them showed the ability to produce urease. 16S rDNA sequencing analysis showed the isolated bacteria belong to genus Bacillus, Virgibacillus, Halobacillus and Staphylococcus. Further studies focusing on the enzymestructures and encoding genes are suggested.

Keywords: Halophiles, urease, screening.