ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access

L. Sharma*, U. Biswas, C. Guha, A. Chatterjee, P.S. Jana and R. Pandey

Department of Veterinary Epidemiology & Preventive Medicine, West Bengal University of Animal and Fishery Sciences, 37, K. B. Sarani, Kolkata-700037, India.
J Pure Appl Microbiol. 2017;11(2):981-986
https://doi.org/10.22207/JPAM.11.2.40 | © The Author(s). 2017
Received: 25/09/2016 | Accepted: 03/12/2016 | Published: 30/06/2017
Abstract

Vaccine was prepared by using the isolate, exposed to 430C for 12 days. The vaccination was performed in two thousand four hundred breeder flocks (Banoraja) in 3 different flocks @106.5 EID50 per bird oronasally at the age of day 5 (primary vaccination) followed by vaccination through drinking water on day 26 (boostering) in farm  condition and thereafter every 45 days interval. The vaccine was also inoculated intranasally @106.5 EID50 in Two thousand backyard birds (Haringhata Black and non-descriptive) and were boostered after 21 days of 1st vaccination with the same dose and subsequent vaccination was performed at every 45 days interval. Serum samples were collected for HI titre at regular intervals and it was observed that the mean antibody both in backyard birds and breeder flocks was above 1.50 ± 00. Therefore, it was concluded that the thermostable live attenuated lentogenic strain Newcastle Disease vaccine after inoculation into backyard birds and breeder flocks, provides satisfactory level of immunity.

Keywords

Vaccine, Chicken, Newcastle, Serum sample

Article Metrics

Article View: 1344

Share This Article

© The Author(s) 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.