ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Garima Verma1 , Nadim Chishty1 and Chandra Veer2
1Department of Zoology, Government Meera Girls College, Mohan Lal Sukhadia University, Udaipur – 313 001, Rajasthan, India.
2Department of Dairy & Food Microbiology, College of Dairy & Food Science Technology, MPUAT, Udaipur, Rajasthan, India.
J Pure Appl Microbiol. 2017;11(2):975-979
https://doi.org/10.22207/JPAM.11.2.39 | © The Author(s). 2017
Received: 10/12/2016 | Accepted: 10/01/2017 | Published: 30/06/2017
Abstract

The present study deals with isolation and characterization of lead resistant bacteria isolated from two water bodies Udaisagar lake and Gadwa pond of Berach river system, Udaipur, Rajasthan, India. Initially, among 13 of the total isolates screened from water samples, 2 isolates were selected for study based on high level of heavy metal resistances. On the basis of morphological, biochemical and molecular characterization using 16S rDNA sequencing the isolates were identified as Pseudomonas stutzeri (KX692284) and Pseudomonas stutzeri (KX692285). The isolates exhibited high resistance to lead (Pb). The Minimum Inhibitory concentration (MIC) of the isolates against lead was determined using agar plate dilution method. Pseudomonas stutzeri showed highest MIC value for lead up to 1300 mg/l concentration. The uptake of heavy metals, present in water and detoxification of metal ions by bacteria provide an additional mechanism of environmental bioremediation. The identified lead resistant bacteria could be useful for the bioremediation of lead contaminated sewage and waste water from various industries.

Keywords

Pseudomonas stutzeri, Lead Resistant Bacteria, Minimum Inhibitory concentration (MIC), bioremediation, 16S rDNA sequencing.

Article Metrics

Article View: 1424

Share This Article

© The Author(s) 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.