Microbial sources for phospholipase A₁ (PLA₁) are economic and industry relevant for degumming of oils and also their product lyso-phospholipids have been widely used as emulsifying agent. Numerous PLAs₁ have been reported, but still the few enzymes have got position in the commercial sector. Due to enormous demand of PLA₁ in the industrial sector, the present study was carried out to optimize PLA₁ production using cheaper agro-industrial waste like defatted rice bran. For this, defatted rice bran was used in solid state fermentation for the production of PLA₁.One-factor at a time approach was used to obtain maximum production of 51.5 U/gm which is 2.15 folds more than un-optimized medium. The optimized medium components were (pH 7): defatted rice bran (5gm), glucose (1% w/v), peptone (1% w/v) and olive oil (0.5 % v/v) with moisture content of 1:1.5 and after 48h of incubation at 37°C. This approach will provide the cleaner solution for degumming of oil as compare to the acid degumming and also help to reduce the stress of the environment by utilizing waste. This is the first report where in Bacillus subtilis subsp. inaquosorum was employed for PLA₁ production via solid state fermentation.