Isolation, Identification and Antibiotic Sensitivity Profiling of Methicillin Resistant Staphylococcus aureus from Bovine Milk in Bihar

ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access

Isolation, Identification and Antibiotic Sensitivity Profiling of Methicillin Resistant Staphylococcus aureus from Bovine Milk in Bihar

Mausam¹, P.K. Ray¹, A. Dey¹, S. Mohanty¹, P. Kaushik², Anjay², Manju Sinha³ and S. K. Gupta¹

¹ICAR-Research Complex for Eastern Region, PO:BV College, Patna – 800 014, Bihar, India.

²Department of Veterinary Public Health, Bihar Veterinary College, Patna – 800 014, Bihar, India.

³Institute of Animal Health and Production, Patna – 800 014, Bihar, India.

J Pure Appl Microbiol. 2016;10(4):3183-3188

https://doi.org/10.22207/JPAM.10.4.95 | © The Author(s). 2016

Received: 24/08/2016 | Accepted: 27/09/2016 | Published: 31/12/2016

Abstract

The aim of this study was to know the prevalence of Staphylococcus aureus in bovine milk samples and to evaluate the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) among S. aureus isolates. A total of 150 bovine milk samples were analysed which showed the prevalence of S. aureus in 56.67% of samples. The mecA gene detection by PCR among S. aureus revealed the distribution of MRSA in 29.33% of bovine milk samples. Antibiotic sensitivity pattern reveals that 86.36 and 95.45 percent MRSA isolates were resistant to penicillin and ampicillin respectively, whereas 80.49 and 87.80 percent non-MRSA isolates were found to be resistant to penicillin and ampicillin antibiotics respectively.

Keywords

Staphylococcus aureus, bovine milk, MRSA, mecA and ABST

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