RNA-guided Genome editing tool CRISPR-Cas9: its Applications and Achievements in Model and Crop Plants

Om Prakash Patidar1*, Chirag Gautam2, Girish Tantuway3, Sunil Kumar1, Ashok Yadav1 , Dharam Singh Meena2 and Arvind Nagar1

1 Indian Agricultural Research Institute, New Delhi, India. 2University of Agricultural Sciences, Bengluru, Karnataka, India. 3Banaras Hindu University, Varanasi, Uttar Pradesh, India.

Received on 06 July 2016 and accepted on 18 September 2016

 

ABSTRACT

Genome editing through efficient and versatile bacterial immunity based CRISPR Cas9 system has revolutionized the molecular biology. Every day new achievements in basic and applied research using CRISPR are coming in. Here, we review the some of the important advancements in methods to increase its efficiency in site-specific genome editing and achievements in crop improvement researches. It is expected from this review that it will provide recent updates on the CRISPR-Cas9 system and will help users involved in applied researches in plant biology to make them exploit the full potential of this genome editing tool.

Keywords

 CRISPR/Cas9, Genome editing, CRISPaint, DSB, Transgene-free, mushroom.