Isolation, Partial Purification and Assessment of the Antimicrobial Activity of a Peptide from the Flowers of Catharanthus roseus (White Flower) and (Pink Flower)

Mansi Srivastava¹, Sushma Ahlawat²and Manoj Kumawat3

Department of Biochemistry and Biochemical Engineering, SamHigginbottom Institute of Agriculture, Technology and Sciences, Allahabad, India.

Received on 18 May 2016 and accepted on 23 June 2016

 

ABSTRACT

Extraction, isolation, and purification of an antimicrobial peptide was carried out from the flowers of Catharanthus roseus (white flower) and (pink flower) varieties and their antimicrobial activity was monitored against bacteria Escherichia coli( Gram-negative)and Staphylococcus aureus (Gram-positive).Cathranthusroseus is well known for its medicinal properties. This study illustrates the antibacterial property of this plant against pathogenic and opportunistic organisms like Escherichia coli and Staphylococcus aureus.The results showed that the Catharanthusroseus flowers (white)and (pink) purified extracts have antibacterial activity against Escherichia coli and Staphylococcusaureus. Therefore providing protection to the flowers against pathogen invasion. This study also shows that an antibacterial compound has been isolated and detected.

Keywords : 

Catharanthus roseus; Peptide; Antimicrobial; Purification.