Assessment of Immune Response Against Newcastle Disease Oral Pellet Vaccine in Desi Chicken by ELISA Test

T. Lurthu Reetha1, J. Johnson Rajeswar2, T.J. Harikrishnan3, K. Sugumar4, P. Srinivasan5 and J.John Kirubaharan6

1Tamilnadu Veterinary and Animal Sciences University, Regional Research,Pudukkottai – 622004, India 2.Department of Veterinary Microbiology, Veterinary College andResearch Institute, Tirunelveli-627001,India 3Directorate of Research,Tamilnadu Veterinary and Animal Sciences University Chennai -600051,India. 4Department of Veterinary Microbiology,Veterinary College and Research Institute, Namakkal -637002, India 5Veterinary University Training and Research Centre,Nagapattinam,Tamilnadu, India. 6Department of Veterinary Microbiology,Madras Veterinary College, Chennai – 600007, India.

Received on 26 May 2016 and accepted on 11 July 2016

 

ABSTRACT

The study was carried out at Veterinary University Training and Research Centre, Tiruchirapalli, Tamil Nadu. A total of 48 day old desi chicks obtained from a private hatchery in Namakkal, TamilNadu were maintained under cage system of rearing up to 52 weeks of age as per standard management practices. All the 48 chicks were divided into six groups having eight chicks in each group were subjected to different treatment regimes. Serum samples were collected at 21 days interval and the post vaccination antibody titre was assessed by ELISA tests. All the birds were challenged at 52 weeks of age with 0.5 ml dose of 104.0 EID50 virulent ND field virus. All the vaccinated groups in this study showed protective level of antibody titre throughout the study period of 52 weeks. The challenged birds were observed for ten days for the development of clinical symptoms, lesions and mortality. No mortality was observed in any of the vaccinated group whereas 100 percent mortality was recorded during the observation period in the unvaccinated control groups.

Keywords :

 Oral pellet vaccines, desi chicken, ELISA test, immune response.