In this present studied poly bag experiment was conducted following complete randomized block design with 12 treatments and three replications. Polluted Soil with supply of fresh water, Unpolluted soil with supply of fresh water, Unpolluted soil with supply of polluted water. The results of pot culture were reveals that the Influence of microbial cultures on biological quality and microbial population of polluted soil and spinach yield at 30 and 60 DAS was estimated. Significantly highest bacterial population was recorded in treatments T₁₂ (124.21 ×10⁷ CFU g^-1 soil) at 30 DAS and treatment T₈ (88.68 ×10⁷ CFU g^-1 soil) at 60 DAS. The highest molds population was observed in treatment T₃ (17.91, 11.32 ×10³ CFU g^-1 soil) at 30 and 60 DAS respectively. The treatment T₆ showed significantly highest rhizobial population at 30 and 60 DAS (29.23, 20.71 ×10³CFU g^-1 soil) respectively. The VAM population was highest in the treatment T₇ (24.13 ×10 g^-1 soil) at 30 DAS and T₁₂ (9.0) at 60 DAS. The highest leaf fresh weight was recorded in T₈ (41.63 g plant^-1) at 30 DAS and (70.03 g plant^-1) at 60 DAS and the lowest fresh weight was recorded in T₃, T₉ at 30, 60 DAS respectively. The highest leaf dry weight was recorded in T₈ (6.62 g plant^-1), (4.17 g plant^-1) at 30 and 60 DAS respectively and the lowest values were found in T₃ at 30 and 60 DAS.