The use of alternative substrates for biomass production has advantages for the production of non-conventional sources of protein and functional products. Qualitative and quantitative differences were determined in two culture media for the production of biomass by Pleurotus djamor (Rumph. : Fr.) Boedijn (P-19) and P. ostreatus (Jacq. : Fr.) Kumm. (P-11). Growth rate and biomass yield increased when non-conventional culture media were used. In liquid culture, the specific growth rate (µ) for the strain P-19 on Liquid Extract of Malt (EML+BF) medium was of 0.196 h^-1 and 0.403 h^-1 for the strain P-11, while on Buffer of Liquid Phosphates added with cereal (BFL+C) medium it was of 0.233 h^-1 for the strain P-19 and 0.395 h^-1 for the strain P-11. There were significant differences among strains studied. The production of mycelial biomass was of 0.07 g/l in EML+BF medium and 0.49 g/l in BFL+C medium for the strain P-19, while for the strain P-11 it was of 0.18 g/l and 0.70 g/l in EML+BF and BFL+C media, respectively. There were significant differences among treatments. The use of substrates containing lignocellulosic material was suitable for biomass production, increasing growth rate.