The field experiment was conducted to access the effect of different fertilizer levels on yield, nutrient content and uptake of seed and straw under middle Gujarat condition during rabi season of 2013-14 at Anand. There are total with three nitrogen levels (15, 30, and 45 kg N ha-1), three levels of phosphorus (0, 15 and 30 kg P2O5 ha-1) and two levels of potassium (0 and 30 kg K2O ha-1) with four replication. The result revealed that successive increasing of nitrogen, phosphorus and potassium levels significantly affect the nutrient content and uptake by seed and straw as well as seed and straw yields of isabgol. Application of 45 kg N ha-1 and 30 kg P2O5 ha-1 gives significantly highest N, P and K uptake and content as well as yields of seed and straw of isabgol. Response of potassium was found to be non-significant in case of nutrient content, uptake and yield of isabgol.