ISSN: 0973-7510

E-ISSN: 2581-690X

Jiayi Guo1, Jiulong Li2, Gengxiao Man2, Yao Yao3, Yin Wang4, Zhi Lin5 and Weidong Huang2,6
1Research Center of Medical Science and Technology, Ningxia Medical University, Ningxia, 750004, P.R. China.
2Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Ningxia, 750004, P.R. China.
3Department of Chemistry, School of Basic Medical Science, Ningxia Medical University, Ningxia, 750004, P.R. China.
4Department of Neuroscience, School of Basic Medical Science, Ningxia Medical University, Ningxia, 750004, P.R. China.
5Centre for Life Sciences, Department of Physiology, National University of Singapore, 117456. Singapore.
6Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education,
Yinchuan, Ningxia , 750004, P. R. China.
J. Pure Appl. Microbiol., 2016, 10 (3): 2379-2389
© The Author(s). 2016
Received: 02/02/2016 | Accepted: 04/04/2016 | Published: 30/09/2016
Abstract

Cyclic dimeric guanosine monophosphate (c-di-GMP), a small secondary messenger, regulates various bacteria physiological processes at transcriptional, post-transcriptional and post-translational levels. P. aeruginosa PAO1 genome contains multiple c-di-GMP metabolizing genes. Transposon mutant of PA0861, one of the c-di-GMP metabolizing genes, showed increased biofilm formation. Siderophores, mainly pyoverdine and pyochelin from P. aeruginosa, are involved in iron uptake. In current study, mainly microarray and RT-PCR analysis were applied to compare the expression profile between wild type and mutant strains of P. aeruginosa. Our transcriptional profiling analysis indicated that pyoverdine synthesis was upregulated in PA0861 mutant. Through spectrophotometrically comparison, it was further demonstrated that pyoverdine production was increased in PA0861 mutant strain. Our results raise the possibility that c-di-GMP regulates siderophore synthetic genes, and affects biofilm formation. Further investigations will be needed to elucidate the mechanisms.

Keywords

Biofilm formation; Cyclic dimeric guanosine monophosphate (c-di-GMP); P. aeruginosa; Pyoverdine; Siderophores.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.