Isolation and Characterization of OMPs and OMVs of Brucella abortus S19 and Brucella abortus S19 per

ISSN: 0973-7510

E-ISSN: 2581-690X

Isolation and Characterization of OMPs and OMVs of Brucella abortus S19 and Brucella abortus S19 per

Khushal Singh Solanki¹ , Dheeraj Pal¹, Gurpreet Kaur²,Pawan Kumar³, Monalisa Sahoo³ and Pallab Chaudhuri²

¹Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh – 243122, India.

²Division of Bacteriology & Mycology, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh – 243122, India.

³Division of Pathology, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh – 243122, India.

J. Pure Appl. Microbiol., 2016, 10 (3): 2121-2126

Received: 12/06/2016 | Accepted: 20/07/2016 | Published: 30/09/2016

Abstract

Bovine brucellosis is a global zoonotic disease. Outer membrane vesicles (OMVs) and outer membrane protein fractions (OMPs) were derived from Brucella abortus S19 , a widely used vaccine strain and it’s perosamine synthetase gene (per) mutant also called Brucella abortus S19Dper using differential density ultrcentrifugation followed by their characterization through sodium dodecyl sulfate polyacrylamide gel electrophoresis and both showed immuno-reactivity with mice antisera in immunoblot. Transmission electron microscopy revealed the presence of OMVs as spherical nano- sized structures.

Keywords

Brucella abortus S19, Brucellosis, Perosamine synthetase gene, Outer membrane vesicles, outer membrane proteins.

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