ISSN: 0973-7510

E-ISSN: 2581-690X

Nahid Rahimifard1 and Mina Naseri2
1Department of Microbiology, Food and Drug Control Laboratories(FDCL), Food and Drug Laboratories Research Center(FDLRC); Ministry of Health(MOH), Iran.
2Department of Microbiology, Islamic Azad University,Saveh, Iran.
J. Pure Appl. Microbiol., 2016, 10 (3): 1885-1889
© The Author(s). 2016
Received: 02/04/2016 | Accepted: 23/05/2016 | Published: 30/09/2016
Abstract

During the last decades, the prevalence of foodborne diseases due to contaminated food as well as demand for natural and healthy foods has increased. Using probiotics for this purpose and for inhibiting growth of food pathogens is an interesting topic. The aim of this study was  to investigate the antibacterial effects of Bifidobacterium bifidum and Bifidobacterium infantis against salmonella enterica serotype Enteritidis by three different methods  namely spot on lawn assay, agar well diffusion assay and agar disk diffusion assay. Supernatant and sediment of the two probiotic bacteria culture was tested in three different assays (spot-on-lawn, well diffusion and disk diffusion) against salmonella. Results showed that in all three assays, sediment and supernatant of Bifidobacterium infantis culture had a greater inhibition effect on salmonella than Bifidobacterium bifidum but the difference was not significant from statistical analyses point of view. In this study in all three methods, the supernatant was significantly more effective than sediment in inhibiting the pathogen. This inhibition could be related to metabolites such as Acids, Diacetyle , Hydrogen proxide, Bacteriocins, produced by probiotics.

Keywords

Probiotic, Bifidobacterium, Salmonella, Antagonism.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.