ISSN: 0973-7510

E-ISSN: 2581-690X

Soheir S. Abd El-Salam1 , F.M. Ghaly2, Dina M. Baraka1, Shahira H. Mahmoud3 and Abeer A. El-Makhzangy3
1Department of Botany, Faculty of Science, Benha University, Egypt.
2Department of Botany, Faculty of Science, Zagazig University, Egypt.
3Animal Health Research Institute, Zagazig University, Egypt.
J. Pure Appl. Microbiol., 2016, 10 (3): 1835-1846
© The Author(s). 2016
Received: 12/05/2016 | Accepted: 22/06/2016 | Published: 30/09/2016
Abstract

This study was carried out for isolation and identification phenotypically morphological and biochemical characteristics of bacteria infected Clarias gariepinus and genotypically by Random Amplified Polymorphic DNA (RAPD) also, its susceptibility to different antibiotics and some natural plant extract. A total 289 strains of bacteria isolates , the number of bacterial isolates differed according to the organ of isolation . The highest rate of isolation was obtained from liver (67 isolates) followed by skin (62), intestinal contents (55), kidney (47) and spleen (46) while the lowest number of isolated bacteria were recovered from gills (12). The most commonly isolated bacteria were Aeromonas Sorbia (46.7%), A.caviae (19%), A.jamdaei (8.6%), A.veronii (8.6%), Ps.aeruginosa (2.4%), Ps.fluorescens (2%), Staph.aureus (2.7%), Staph.epidermidis (1%), Vibrio alginolitcus (4.5%) and V.anguillarum (4.15%). All tested isolates were sensitive to Ciprofloxacin followed by Cefuroxime then Gentamycin, the lowest (MIC) and (MBC) were recorded for Ciprofloxacin and alcoholic Clove extract among the plant species tested, Cinnamon, Hibiscus, Nutmeg, Thymus and Rosemary against all bacterial isolates.

Keywords

Antibiotic susceptibility, Herbal sensitivity, Aeromonas spp., Phenotypic, Genotypic, Catfish.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.