Antimicrobial resistance, the ability of bacteria to inhibit the function of antibiotic drugs, has become a public health concern. To investigate susceptibilities of common clinical strains to antimicrobials, 1641 clinical isolates were tested according to Clinical and Laboratory Standards Institute (CLSI) guidelines by an annual Jinan Central Hospital in north China surveillance study (JNCHSS) in 2012. Of which, gram positive strains and gram negative strains accounted for 29.3% and 70.7%, respectively. The prevalence of methicillin-resistant strains was 47.0% in S. aureus (MRSA) and 69.8% in coagulase-negative Staphylococcus (MRCNS). No Staphylococcus strain was found to resist to vancomycin or linezolid. In Enterobacteriaceae, extended spectrum b-lactamases (ESBLs) were produced in 60.5% of the E. coli strains and 44.7% of the K. pneumoniae. More than 30% P. aeruginosa strains were only resistant to ticarcililin-clavulanate, aztreonam, imipenem and more than 45% of A.baumannii strains were resistant to all antimicrobials except minocycline. S.maltophilia strains were relatively susceptible to sulfamethoxazole-trimethoprim (SXT), minocycline, levofloxacin with lower resistance rate. There were 54.3% of H. influenzae which produced b-lactamase. In conclusion, as these data clearly illustrate, we are facing a overwhelming situation of bacterial resistance. Much attention should be paid to periodic surveillance of antibiotic resistance, which is necessary and valuable for antimicrobial therapy. Our research provides a summary of antimicrobial resistance in Jinan Central Hospital.