The vast majority of research articles on physiology of macrofungi have used heterokaryons obtained from fruit body tissues. Actually there is evidence that heterokaryons and homokaryons exhibit differences in performances such as mycelium growth rate and properties like colony morphology etc. Ten homokaryons were obtained from a single spore germinated in 2% Malt Extract Agar medium, and their hyphal extension rates on %2 Malt Extract, Potato Dextrose, Hagem and Minimal Agar media were measured. Some significant differences were found in the mycelium growth rates of commercial Hericium erinaceus. Mycelium growth of only one homokaryon could reach to 90 mm diameter on the 27^th days of incubation.  Nine heterokaryotic mycelia formed at the pairing test performed on Potato Dextrose Agar.