ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Nuzhath Fatima1 , Mohd Mazhar Uddin Khan2, Noha Emad3, Khadija Al-Khadir1,4, Amany Marwan3 and Ayesha Alvi5
1Department of Microbiology, Rayalaseema University, Kurnool, Andhra Pradesh, India.
2Faculty of Natural Sciences, Department of Environmental Science, Addis Ababa University, Addis Ababa, Ethiopia.
3Faculty of Applied medical College, Jazan University, K.S.A.
4Department of Microbiology, Mumtaz PG College, Hyderabad India.
5Former Faculty in Department of Genetics, Applied Medical College Jazan University, K.S.A.
J Pure Appl Microbiol, 2019, 13 (3): 1635-1644 | Article Number: 5706
https://doi.org/10.22207/JPAM.13.3.37 | © The Author(s). 2019
Received: 02/06/2019 | Accepted: 28/07/2019 | Published: 08/08/2019
Abstract

The enzyme L-asparaginase is commonly used in treatment of acute lymphoblastic leukemia (ALL). Due to its therapeutic importance and growing demands in medical and food industry much of the research have been focused to produce this enzyme with better activity and less disadvantages; for instance microbes have been extensively used to produce L asparaginase at affordable prices, a wide range of bacterial species have been reported that can produce high yields of this enzyme. The present study is an attempt to compare the production of L asparaginases between gram positive and gram negative bacterial species isolated from soil under same fermentation conditions. In this study the productions of L asparaginase from three species of Bacillus namely Bacillus subtilis (LC425423), Bacillus aerophilus (LC425427), Bacillus endophyticus (MG928501) and three strains of Pseudomonas aeruginosa (LC425424, LC425425, LC425426)  was compared. The enzyme activity of L asparaginases produced from Bacillus species were noted as 8.5, 14.3, 7.1 (U/ml). Whereas, the enzyme activity of L asparaginase produced from Pseudomonas aeruginosa strains (LC425424, LC425425, LC425426) was noted as, 8.2, 19.4, 19.1(U/ml) respectively. In this study it was found that Pseudomonas aeruginosa strains yields was more in terms of protein concentration and enzyme activity when compared to Bacillus species. In addition this study also reports the use of modified Brain heart infusion media for screening and isolation of L asparaginase producing bacterial species. It was found that the number of colonies producing L asparaginase in modified Brain heart infusion media was comparatively same when compared to modified M9 media which is extensively used in screening of L asparaginase producers. A total of 159 L asparaginase producers were screened by using two fold dilution technique on modified BHI media while modified M9 media screened 163 colonies producing L asparaginase.

Keywords

Brain heart infusion media (BHI), ALL (acute lymphoblastic leukemia), L asparaginase (ASP).

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.