Journal of Pure and Applied MicrobiologyVol. 9 No. 3

qPCR detection of Rhodobacter sphaeroides Phage in the CoQ10 Production

Kejun Li1*, Haina Wang2 and Ruiping Zhang1

1 Lab of Space Microbiology, Center of Space Biology Research, China Academy of Space Technology, Peking, China. 2Institute of Microbiology, Chinese Academy of Science, Peking, China.

Received on 26 April 2015 and accepted on 05 June 2015

 

ABSTRACT

Coenzyme Q10 is industrially produced by fermentation and extraction from Rhodobacter sphaeroides. However, phage pollution is a fatal phenomenon in the fermentation industry. The infection of R. sphaeroides by phase was reported for the first time in this study. Based on the obtained genetic sequence from the phage, a quantitative polymerase chain reaction (qPCR) phage detection profile was developed for early detection during the pilot culture to prevent phage pollution in the production lines.

Keywords : Rhodobacter sphaeroides; phage pollution; phage detection; qPCR.