Journal of Pure and Applied MicrobiologyVol. 10 No. 1

Identification and Characterization of Biosurfactant Producing Bacteria Arthrobacter sp. P2(1)

Fatimah1,2*, Suharjono2, Tri Ardyati2, Ni?matuzahroh1, Afaf Baktir3 and Ahmad Thontowi4

1Department of Biology, Faculty of Science and Technology, University of Airlangga, Indonesia. 2Biology Doctoral Program, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Indonesia. 3Department of Chemistry, Faculty of Science and Technology, University of Airlangga, Indonesia 4 Research Center for Biotechnology, LIPI, Cibinong, Indonesia

Received on 11 November 2015 and accepted on 09 January 2016

 

ABSTRACT

The aims of this research were to identify and characterize bacteria isolated from petroleum contaminated soil, P2(1) isolate. This isolate was able to grow on glucose containing medium and produce biosurfactant. This isolate was identified by morphological, physiological and biochemical characteristics and phylogenetic analysis by 16S rDNA sequences. Morphological characteristics observed were colony and cell morphology. Physiological characteristics were observed by microbact kit 12A, 12B. Phylogenetic tree was constructed by comparing 16S rDNA sequences. The results showed that P2(1) isolate was classified into Genus Arthrobacter based on some characteristics, Gram negative in young culture and Gram positive in old culture, rod coccus cycle, and has cream colony on Nutrient Agar medium. The phylogenetic analysis of 16S rDNA sequences showed that P2(1) isolate has similarity with Arthrobacter sp. XBGRY2 (HQ891968.1) with 95% similarity. It can be concluded that the isolate was identified as Arthrobacter sp. P2(1) (KU361211).

Keywords : Arthrobacter sp. P2(1), characteristics of isolate, phylogenetic analysis, biosurfactant.