The aim of the present study was to investigate the effect of orally administrated probiotics on the physiological and immunological parameters of Sprague Dawely rats. To improve the efficiency of some local probiotics, dairy products were used as an excellent delivery system for probiotics to the experimental animals. The experimental animals were divided into seven different groups. It was remarkably noticed that the administration of different probiotics results in a significant increase in body weight gain in all feeding rats compared with the control group. Feeding rats with different probiotics led to increase in Hb and PVC in all groups of rats. On the other hand, a significant increase in RBCs in all feeding rats. An increase in the count of both white blood cells and lymphocytes occurred in all feeding rats. A general reduction in ALT, AST and bilirubin was observed in plasma of all feeding rats. A significant decrease was observed in creatinine level of all feeding rats. Concerning urea, a significant reduction in urea level was found in plasma of all feeding rats. Feeding rats with these probiotics showed improvement in lipid metabolism. A significant reduction in cholesterol level was observed in plasma of all feeding rats. An effective decrease in the level of TG, LDL and VLDL and increase in the level of HDL was observed in plasma of all feeding rats compared with the control group. Administration of probiotics showed significant increase in total serum protein especially globulin compared with control group. ELISA analysis showed the presence of marked variation in immunoglobulins level of all feeding rats compared with control group.