Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Study on Bioactive Property of Marine Bacteria Isolated from Soil Sediment along Vasai Coast

Mukesh R. Pimpliskar and Rahul N. Jadhav

Vidyavardhinis, Zoology Research laboratory, E.S.A. College of Science, Vasai Road, Dist-Palghar, India.

Received on 11 October 2014 and accepted on 28 December 2014

 

ABSTRACT

In the present investigation 9 marine bacteria were isolated and found to be positive during primary screening. Initially the isolates were named as SS1to SS9(soil sediment isolates1 to 9). Among them two organisms SS5 and SS9 were found to be exhibiting broad spectrum antibiotic activity and were subjected to secondary screening. In secondary screening it is found that this organism demonstrated antimicrobial activity against Gram positive,Gram negative, a fungi Candida albicans along with the MRSA (methicillin resistant staphylococcus aureus.). Production of antibacterial and antifungal metabolite were extracted by solvents (chloroform, ethyl acetate, acetone and methanol).Ethyl acetate and methanol and acetone extract of SS5 gave activity against all the test organisms while SS9 gave prominent zone of inhibition in ethyl acetate extract only. The isolates growth parameters were studied and it was found that they grow well at 28?2?C and pH 8. After characterization it was found that the active marine bacteria SS5 and SS9 belongs to Vibrio neocaledonicus.

Keywords : SS5, SS9, broad spectrum, MRSA, zone of inhibition.