Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Profiling of Antibiotic Resistance of Staphylococcus aureus Obtained from Mastitic Milk of Cattle and Buffalo

Rahul Yadav1*, Sandeep Kumar Sharma2, Jyotika Yadav3, Sneha Chaudhary1 and Anil Kumar Kataria1

1Department of Veterinary Microbiology and Biotechnology. College of Veterinary and Animal Sciences, Bikaner. Rajasthan University of Veterinary and Animal science, Bikaner-334001 (Rajasthan), India. 2Department of Veterinary Microbiology and Biotechnology. Post graduate institute of veterinary education and research, Jaipur. Rajasthan University of Veterinary and Animal science, Bikaner-334001 (Rajasthan), India. 3College of Veterinary and Animal Sciences, Hisar. Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar-125001 (Haryana), India.

Received on 02 February 2015 and accepted on 13 March 2015

 

ABSTRACT

The present study was carried out to evaluate antibiotic susceptibility and beta-lactam antibiotic resistance of Staphylococcus aureus isolates from mastitic milk of cattle and buffalo.In the present investigation S. aureus isolates were subjected to antibiogram studies using 33 antibiotics belonging to different categories and generations. Antibiotics such as doxycycline, gentamicin, methicillin, netiline, rifampisin and tobramycin were 100% effective against all isolates from both cattle and buffalo. In case of resistance pattern, maximum resistance was exhibited against nalidixic, cephalexin, polymixin B, cefexime, cephalexin and amoxycillin from both species respectively. The detection of beta-lactam antibiotic resistance was carried out by acidimetric method resulting that only two (12.5%) out of 16 isolates from cattle mastitic milk and nine (56.2%) out of 16 isolates from buffalo mastitic milk were detected as beta-lactamase producer. The analysis of the antibiogram revealed that the susceptibility and resistance shown by the isolates was dependent on use of the antibiotics. The antibiotics of initial generations showed lower efficacies than the antibiotics of latest generations.

Keywords : Staphylococcus aureus, Cattle, Buffalo, Mastitis, Antibiogram.