Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Evaluation of Kinetic Pathway for the Biodegradation of Congo red using Asperigillus species and Optimization of Physiochemical Parameters by Response Surface Methodology

Sathish Sundararaman*, D. Joshua Amarnath, S. Sivasubramanian

Department of Chemical Engineering, Sathyabama University, Rajiv Gandhi Road, Chennai 600 119, India.

Received on 26 March 2015 and accepted on 04 May 2015



Decolorization of Congo red dye was investigated by using dual microbial organism isolated from textile effluent. The effective fungal strain with dye degrading ability was isolated from the effluent treatment plants and was identified as Asperigillus Terreus and Asperigillus Fumigates based on morphological and 16SrDNA sequencing. Analysis for identification of intermediate compounds confirmed the presence of quinone compounds, salicylic acid and catechol, based on this possible pathway for the degradation of dye was proposed. Optimization of temperature, pH, initial concentration of dye and time was investigated by using Response Surface Methodology (RSM). Central Composite Design (CCD) was carried out with 30 experiments, nearly 98.70% of decolorization was observed at 35?C, pH 7.0 and at the initial concentration of 500 mg/l in 72 h. The values of R2 = 0.9924, adjusted R2 = 0.9811 and predicted R2 = 0.9961 which reveals that experimental values are in good agreement with predicted values.

Keywords : Dye degradation, Optimization, Response Surface Methodology, Central Composite Design.