Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Study on Histopathological Changes Induced by Fowl Pox Virus (FPV) in Chorioallantoic Membrane (CAM) of Developing Chicken Embryo and Immunological Characterization of the Egg Adapted and Cell Culture Grown Virus

R.K. Verma1, R.K. Joshi2, N. Joshi3 A. K. Gangwar4 and S. Shukla5

1Department of Veterinary Teaching Veterinary Clinical Complex (T.V.C.C), College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad (U.P.), India. 2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad (U.P.), India. 3Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad (U.P.), India. 4Department ofVeterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad (U.P.), India. 5Department of Biosciences, Integral University, Lucknow (U.P.) 226026, India.

Received on 10 January 2015 and accepted on 02 March 2015

 

ABSTRACT

Histopathological examination was conducted on the chorioallantoic membrane (CAM) of the embryonic chicken eggs (ECE) infected with a field isolate of the fowl pox virus (FPV). In infected CAM, the ectodermic cells appeared swollen and contour of the cells appeared rounded. Some of the cells revealed large vacuole while, other showed increased granularity of the cytoplasm and blood vessels appeared dilated at 6th passage. Ectoderm layer revealed hydropic degeneration with eccentric deposition of nucleus and formation of acidophilic intracytoplasmic inclusion bodies. At 10th passage mesoderm appeared intensively edematous with heavy infiltration of hetrophils and erythrocytes. Presence of FPV antigen could be easily detected in tissue sections. The antigen was mostly localized in the perinuclear area in the cytoplasm of the infected cells and the area showing intracytoplasmic inclusions were easily appreciable.To confirm the replication of virus in the CAM and cell culture, the 10% (w/v) suspensions of infected CAM and cell culture supernatants were subjected to Enzyme linked immune sorbent assay (ELISA) for detection of FPV antigen using antigen capture ELISA. The comparison of ELISA titers indicates that virus yield was better on CAM as compared to BGM-70 cells.

Keywords : Fowlpox virus (FPV), Chorioallantoic membrane (CAM), Pock lesions, Acidophilic intracytoplasmic inclusion bodies, BGM-70 cells.