Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Screening of Lactic Acid Bacteria as Biocontrol against Colletotrichum musae

M. Ashwini1*, Archana1, Shamarao Jahagirdar2, G.S. Geeta1 and Smita R. Babar1

1AICRP on RES (Bioconversion Technology), Main Agriculture Research Station, University of Agricultural Sciences, Dharwad - 580 005, India. 2Department of Plant Pathology, AICRP on Soybean, University of Agricultural Sciences, Dharwad - 580 005,India

Received on 13 February 2015 and accepted on 11 March 2015

 

ABSTRACT

In the preliminary study, three hundred and seventy six Lactic Acid Bacterial (LAB) isolates obtained from the various natural sources were morphologically characterized and screened against the fungal pathogen Colletotrichum musae, causal agent of anthracnose of banana. The dual culture assay was employed to determine in vitro antagonistic activity of the LAB isolates. Among these twenty four, LAB isolates inhibited the mycelial growth of the pathogen. Out of twenty four, only two isolates viz. LS-36 and LS-44 exhibited maximum zone of inhibition. Based on 16S rRNA gene sequencing, LS-36 and LS-44 revealed maximum similarity to Lactobacillus brevis.

Keywords : Biocontrol, Lactic acid bacteria, Anthracnose, Colletotrichum musae.