Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Optimization and Chemical Characterization of Exopolysaccharide produced by Pseudomonas sp. Isolated from Wheat Rhizosphere

Devendra Singh1, Manjunatha B.S2, Sukumar Taria3 and Geeta Singh*

1,2Division of Microbiology, Indian Agricultural Research Institute, New Delhi- 110012, India. 3Division of Plant Physiology, Indian Agricultural Research Institute New Delhi- 110012, India.

Received on 20 December 2014 and accepted on 15 February 2015

 

ABSTRACT

Enrichment technique was implemented to isolate and detect osmotolerant EPS-producing bacteria from the rhizosphere of wheat growing under semi arid tropical soil. The highly osmotolerant isolate, identified as a Pseudomonas species by 16S rDNA analyses, produced EPS, as evidenced by colorimetric and gas chromatographic analyses. Quantitatively the EPS production was influenced by the osmotic stress. Exopolysaccharides (EPS) produced from Pseudomonas were studied as affected by different carbon, nitrogen sources, C:N, pH of culture media. The FT -I.R. and GC-mass spectroscopy data of purified polysaccharides from the organism revealed presence of many functional groups in the EPS.

Keywords : Exopolysaccharides, Pseudomonas, C: N, carbon, FT -I.R., GC-mass spectroscopy.