Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Regulation of a-amylase Activity and Blood Glucose Levels in Diabetic Sprague-Dawley Male Rats by the Aqueous Extract of Andrographis paniculata

Sangeetha Sundaramoorthy*, Archit Rastogi, Sathiavelu Mythili and Sathiavelu Arunachalam

1SBST, VIT University, Vellore ? 632014, India.

Received on 29 March 2015 and accepted on 04 May 2015

 

ABSTRACT

Hypoglycemic effect of Andrographis paniculata leaf extract and its glucose uptake inhibition effect in rat invivo models has been studied.Hypoglycemic studies were carried out in glucose loaded and starch induced diabetic albino rats.Blood glucose levels were estimated at 10, 20, 30, 60, 120, 240 and 360 min after administration of methanolic extract of A.paniculata by using One Touch digital glucose reader in both 200 and 500 mg/kg body weight.The blood glucose levels were significantly depleted in the animals administered with aqueous leaf extract of A.paniculata in both 200 and 500 mg/kg body weight. The aqueous extract of A. paniculata is very effective at regulating the activity of a-amylase and regulating blood glucose levels. There was a dose-response decrease in glucose absorption by the extract. The leaf extract of A. paniculata has in vivo efficacy for inhibition of starch absorption and may prove beneficial in weight reduction in individuals consuming large amounts of starch. It also may inhibit starch-induced hyperglycemia in normal and diabetic subjects.

Keywords : A. paniculata, Dose-dependent, a-amylase,Starch.