Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Bacterial Community Diversity and their Composition in Different Soil Ecosystems Analyzed by PCR Coupled Culture Independent Methods

Sachin A. More*, Malik Ahmed Pasha, Smita R. Babar and P.U. Krishnaraj

Department of Agricultural Biotechnology, University of Agricultural Sciences, Dharwad- 580005, Karnataka, India.

Received on 30 March 2015 and accepted on 08 May 2015

 

ABSTRACT

Exploration of bacterial community diversity linked with different forest and farm soil ecosystems is very essential to get information about their role in the maintenance of respective soil ecosystems. Different parameters associated with ecosystems have significant effect on inhabitance of diverse bacterial communities. Our study deals with, unraveling of bacterial community diversity and their composition in two different kinds of forest and farm soil ecosystems. This was accomplished by using molecular biology methods such as PCR-DGGE and sequencing of 16S rDNA clone library of metagenomes extracted from forest and farm soils. Study revealed that there was high level of similarity (about 77%) in bacterial community among two forest soil and more than 60% of similarity between forest and organic farm soil. However, less than 38% of similarity was seen between forest and degraded farm soil bacterial communities. Further 16S rDNA sequencing results were showed bacterial composition in each type of soil ecosystem. All soil types have shown presence of uncultured microbes with abundance of ?-proteobacterial communities. Apart from g-proteobacteria, other bacterial communities such as Acidobacteria, Actinobacteria, g-proteobacteria and Plactomycetes were found in organic farm soil whereas Firmicutes was present in degraded farm soil. The results of present study demonstrated that, the bacterial diversity observed in this experiment is very less irrespective of soil ecosystem. This reflects that, those may be intensively threatened by biotic/abiotic factors and human activities associated with it. Thus, these forest and farm soils are need to be protected for maintaining ecological balance.

Keywords : Bacterial diversity, Soil ecosystems, Idependent methods.