Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Paraoxonase Q192R Gene Polymorphism and Cardiovascular Risk are Associated with Polycystic Ovarian Syndrome Women

Jayasree Ravindran1*, Vijayalakshmi Melanathuru2 and A. Jamuna Devi3

1Department of Biotechnology, Rajalakshmi Engineering College, Chennai - 602 105, India. 2Department of Biotechnology, Dr. M.G.R. University and Research Centre, Chennai , India. 3Department of Biotechnology, Rajalakshmi Engineering College, Chennai 602 105, India.

Received on 10 February 2015 and accepted on 11 March 2015

 

ABSTRACT

The aim of the study was to investigate the lipid profile and its correlation with paraoxonase enzyme in PCOS women and to identify the association of PON1 192 polymorphism with cardiovascular risk. Human paraoxonase 1 (PON1 EC 3.1.8.1) is a HDL-complexed enzyme. It is of clinical importance due to its established associations with inhibition of LDL oxidation. It has anti-oxidative properties too. A decreased paraoxonase activity may contribute to cardiovascular risk. 53 test and 56 control subjects were included in the study. PCOS women exhibited a variety of symptoms whereas the control subjects were chosen to be regularly menstruating women. Blood samples were collected and used for the assay . DNA samples were used for the genetic studies. Polymorphisms were determined by restriction fragment length polymorphism. The results of the study shows a rise in the levels of cholesterol, LDL cholesterol and a decreased HDL in the study subjects when compared to control. A decreased paraoxonase level reported and the genetic variations identified relate the polymorphism in the gene of paraoxonase, with increased risk for cardiovascular diseases. Paraoxonase Q192R Gene Polymorphism are associated with cardiovascular risk.PON1 seems to be related with hyperlipidemia and cardiovascular risk in women with PCOS.The purpose of the study was to assess the impact of paraoxonase level and its genetic variation in PCOS women. The study also investigated if these women harvest a risk for cardiovascular disease.

Keywords : Paraoxonase, Polycystic ovary syndrome, Polymorphism, genetic variations, cardiovascular risk.