Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Application of Inter Simple Sequence Repeat (ISSR) for Detecting Genetic Analysis in Rice (Oryza sativa L.)

Arshad Naji Alhasnawi1,3*, Ahsan A. Kadhimi1,4, Anizan Isahak2, Mehdi Farshad Ashraf1, Febri Doni1, Azhar Mohamad5, Wan Mohtar Wan Yusoff1 and Che Radziah Che Mohd Zain1*

1School of Biosciences & Biotechnology, Faculty of Science and Technology, UniversitiKebangsaan Malaysia. 2School of Environmental Science and Natural Resources, Faculty of Science and Technology, UniversitiKebangsaan Malaysia. 3University Presidency, AL- Muthanna University, Iraq. 4University of Baghdad, Ministry of Higher Education, Iraq. 5Malaysian Nuclear Agency, Malaysia.

Received on 20 November 2014 and accepted on 12 January 2015



The progress technology of molecular markers to genetic diversity has enabled to an increase in our knowledge of rice genetics and our understanding of the structure and behavior of genomes. DNA markers technology becomes the markers of the study of the genetic analysis of rice species, cultivars and genomes, because they are able to assessment the genetic variation more precisely, cheaply and quickly. These techniques in special the use of molecular marker, have been utilized to study of DNA structure divergence in and during the create new sources of genetic diversity, genetic variation and species by introducing appropriate and new characteristic from related grass species and landraces. Development in molecular marker detection systems and in the techniques used to identify markers linked to useful traits has led large advances to be made in recent years. ISSR markers are highly polymorphic and are useful in researches on phylogeny, genome mapping, evolutionary biology, genetic diversity and gene tagging. This review investigator the use of this techniques for the inter simple sequence repeat (ISSR) fordetecting genetic analysis in rice.

Keywords : Genetic diversity;Genetic marker; ISSR; Rice.