Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Isolation, Biochemical and Virulence Characterisation of Enterococcus faecalis and Enterococcus faecium Isolated from Poultry Feces

Kanchan Jangir, B.N. Shringi, Irfan Ahmad Mir, Ram Hari Meena, Nishtha Purohit, Sunil Maherchandani and S.K. Kashyap

Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner-334001, India.

Received on 10 January 2015 and accepted on 06 March 2015

 

ABSTRACT

The present investigation was conducted to study the prevalence of two important Enterococcus species viz. E. faecalis and E. faecium and some of the selected phenotypic characters associated with virulence. A total of 50 fecal samples were collected from various poultry farms for isolation using a Pfizers Enterococcus agar. Preliminary identification was done by observing the colonial morphology, Grams staining and a set of 12 biochemical tests. Final confirmation was made by species-specific PCR. The biochemical characterization involved subjecting the isolates to a set of 18 different sugars to note their fermenting activity. From 50 samples, a total of 48 isolates were identified to belong to the genus Enterococcus. The species-specific PCR showed that out of 48 Enterococcus sp. isolates, 25 (52.08%) belonged to E. faecalis, 15 (31.25%) were identified as E. faecium while rest 8 isolates were considered as miscellaneous. Biochemical characterization of isolates showed atypical reaction in fermentation of some sugars viz. Adonitol, Arabitol, Dulcitol and inulin. Virulence characterization involved attributes viz. gelatinase-production, caseinase-activity and haemolysin-production. The study showed gelatinase, caseinase and haemolysin production to a level of 84%, 72% and 40% respectively by E. faecalis while 46.66%, 40% and 53.33% were seen by E. faecium isolates respectively. Except haemolysin production all properties were more evident in E. faecalis isolates as compared to that of the E. faecium isolates.

Keywords : Enterococcus faecalis, Enterococcus faecium, Poultry, Virulence, Species-specific PCR.