Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Characterization of Staphylococcus aureus of Goat Mastitis Milk Origin for cap and clfA Genes

Prerna Nathawat1, Taruna Bhati2, Sandeep Kumar Sharma3*, Rahul Yadav2 and Anil Kumar Kataria2

1Centre for Studies on Wildlife Management and Health, College of Veterinary and Animal Science, Bikaner, Rajasthan University of Veterinary and Animals Sciences, Bikaner (Rajasthan), India. 2Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Bikaner Rajasthan University of Veterinary and Animals Sciences, Bikaner (Rajasthan), India. 3Department of Veterinary Microbiology and Biotechnology, Post Graduate Institute of Veterinary Education and Research (PGIVER)- Jaipur, Rajasthan University of Veterinary and Animals Sciences, Bikaner (Rajasthan), India.

Received on 02 February 2015 and accepted on 06 March 2015

 

ABSTRACT

Staphylococcus aureus is major pathogen causing mastitis in dairy milch animals including goats. The present study was carried out on 27 S. aureus isolates of milk origin from goats with clinical mastitis which included characterization of isolates for two important virulence associated genes namely clfA, responsible for clumping factor and cap, responsible for capsule. All the isolates were genotypically identified by 23S rRNA ribotyping in which a species-specific amplicon of 1250 bp was obtained. Of the 27 isolates 17 (68.38%) showed single amplicon of approximately 350 bp representing presence of cap5Kgene responsible for capsular type 5 whereas nine (34.61%) of the isolates produced amplicons of approximately 200bp representing presence of cap8K genes responsible for production of capsular type 8, whereas one isolate was non-typable for any of the two genes.In the present investigation each of the 25 isolates produced single amplicon of 1050 bp indicating no polymorphism in clfA genewhile two isolates did not produce any PCR amplicon. Epidemiological investigation of these genes is significant in terms of vaccine design and control of mastitis from the study area.

Keywords : Staphylococcus aureus, cap, clfA, goat, mastitis.