Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Prevalence of hlg and pvl Genes in Methicillin Resistant Staphylococcus aureus(MRSA) Isolated from Health Care Staff in Mofid Children Hospital,Tehran, Iran

Shahnaz Armin1, Farhad Fareghi2, Fatemh Fallah1, Masoud Dadashi2, Bahram Nikmanesh3 and Zohreh Ghalavand2*

1Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2Department of Microbiology,School of Medicine, Shahid Beheshti University of Medical Sciences,Tehran,Iran. 3Department of Lab Medical Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.

Received on 09 February 2015 and accepted on 19 April 2015

 

ABSTRACT

Methicillin Resistance Staphylococcus aureus(MRSA) is a type of Staphylococci that is resistant to the antibiotics such as methicillin,cloxacillin,dicloxacillin,naficillin and cephalosporins. Thehlgand pvl are two important genes that confer virulence traits in MRSA strains.Gamma-hemolysin is toxicfor human erythrocytes, whereas Panton-Valentine Leukocidin (PVL) is toxic for human and rabbit macrophages and polymorphonuclear(PMNs)cells. In present study, we sought to examine the prevalence of MRSA strains and detect the genes of hlg and pvl in health care staff. The descriptive studywas conductedfromJanuarytoDecember 2014. In this survey,two hundred twenty-ninenose specimens were taken from the health carestaff of Mofid Children Hospital, Tehran.The isolates were identified as S.aureusbased on biochemical and phenotypical tests.To determine the profile of antibiotic resistance of S.aureusisolates,the disk diffusion method (Kirby-Bauer) was used according to 2013 CLSI guidelines.MRSA strains were ascertained by resistance to oxacillin and cefoxitin. The PCR assayswere used for detection of hlg andpvlgenes.PCR product was sequenced and thedata analyzed using SPSS software (version 19).Health care staff included 200 (87.33%) female and 29 (12.66%) male. Out of 229 samples, 27(12%) isolates were positive for S.aureusof which 21 (77.7%) were MRSA and 6 (22.3%) were MSSA (Methicillin Sensitive S. aureus).The results of PCR showed that 18 (85.71%) of MRSA isolates harbored hlggenebut the isolates were negative for the presence ofpvl gene.In conclusion, gamma-hemolysinappears to be a more possible virulence factor than Panton-Valentine Leukocidin in MRSA isolates.

Keywords : Methicillin Resistance Staphylococcus aureus,hlggene, pvlgene, medical staff.