Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Isolation, Classification, Culture Conditions, and Enzyme Production of a White Rot Fungus Cerrena sp. GSM-01

Shan-Shan Wang1,2, Qing-Jun Chen1, Kai Cui2, Zi-Jiang Wang2, Shou-Nan Wang2, Hai-Wen Hu2, and Guo-Qing Zhang2*

1 Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China. 2 Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Sciences and Engineering, Beijing University of Agriculture, Beijing 102206, China.

Received on 23 February 2015 and accepted on 10 April 2015

 

ABSTRACT

As white rot fungi, Cerrena species manifest efficient lignin degrading enzymes for bioremediation applications. A fungal strain from the genus Cerrena was isolated from its fruiting bodies using the tissue isolation method and numbered as GSM-01. It was identified as Cerrena unicolor using ITS identification methods. Optimal culture conditions of mycelia were: favorite carbon source of maltose, favorite nitrogen source of yeast extract, favorite C/N rate of 10/1, favorite growth factor of vitamin B1, optimal temperature of 32 ?C, and optimal pH of 6.5. It demonstrated extremely high laccase activity in liquid fermentation. The highest extracellular laccase activity of 2855.0 ? 41.6 U/mL was obtained in the broth with a Cu2+ adjunction concentration of 1.0 mM after a 8th day of cultivation. It is about 190-fold of that of reported C. unicolor strains and indicated its further applications. It also manifested considerably high RNase and phytase activities, but was devoid of protease and lectin activities.

Keywords : Cerrena unicolor, ITS identification, Laccase, Liquid fermentation, Mycelia.