Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Frequency, Susceptibility and Co-Existence of MBL, ESBL & AmpC Positive Pseudomonas aeruginosa in Tertiary Care Hospitals of Peshawar, KPK, Pakistan

Muhammad Ilyas1, Muhammad Khurram1, Shabeer Ahmad2 and Irshad Ahmad3

1Department of Microbiology, and Biotechnology, Sarhad, University of Science and Information Technology, Peshawar, KPK, Pakistan. 2Department of Microbiology, Abasyn University, Peshawar, KPK, Pakistan. 3Department of Life Sciences King Fahd University of Petroleum and Minerals PO Box 2001, Dhahran 31261, Saudi Arabia.

Received on 12 February 2015 and accepted on 10 April 2015

 

ABSTRACT

Pseudomonas aeruginosa responsible for nosocomial infections prevail in hospitals, producing b-lactamase enzymes that hydrolyze b-lactam drugs and reduce their efficacy. This study was carried out to determine prevalence, susceptibility and production of different b-lactamases by P. aeruginosa. The isolates were screened and characterized using standard protocols. Among isolates 32%, 10%, 6% and 6% were obtained from pus swabs, sputum, urine and body fluids, respectively. Prevalence of MBL positive strains was 25.7%, AmpC was 17.1% and ESBL was 8.5%. The co-production of MBL+AmpC, MBL+ESBL and ESBL+AmpC was 10%, 5.7% and 2.8% respectively. MBL+AmpC positive strains were 100% resistant to ceftriaxone, amoxicillin+clavulanic acid, norfloxacin. ciprofloxacin, cefaperazone+sulbactam, pipracilline+tazobactum, cefotaxime and imipenem, While MBL+ESBL positive stains were 100% resistant to ceftriaxone, amoxicillin+clavulanic acid, norfloxacin, ciprofloxacin, ceftaxime, ceftazidime, imipenem, cefaperazone+sulbactam and pipracilline + tazobactum. Only least resistant (25%) observed for amikacin and cefepime. Co-production of AmpC+ESBL positive strains were 100% resistant to ceftriaxone, amoxcilline+clavulanic acid, ciprofloxacin, norfloxacin and cefotaxime, cepaferazone/sulbactum and pipracilline/tazobactum. Production of MBL was higher than ESBL and AmpC b-lactamase. Co-production of these enzymes was responsible for multidrug resistance. Production of MBL+AmpC was higher than ESBL+AmpC and ESBL+MBL. Least resistance was noted against cefepime and amikacin by MBL, ESBL and AmpC co-producers.

Keywords : Pseudomonas aeruginosa, Metallo b-lactamase, Extended Spectrum b-lactamase, AmpC b-lactamase.