Journal of Pure and Applied MicrobiologyVol. 9 No. 2

The Isolation and Identification of Actinobacillus pleuropneumoniae and Antimicrobial Susceptibility From Pigs in China

Yongjun Dong, Lirong Wang*, Xingyou Liu and Kun Zhao

College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China.

Received on 25 December 2014 and accepted on 01 February 2015

 

ABSTRACT

Seven strains of bacteria were isolated from clinical cases of swine disease in lungs and trachea. The isolated bacteria was identified as Actinobacillus pleuropneumoniae (APP) serotypes 1 through bacterial?cultivated,?culture characteristic observation, morphology, biochemical, growth of satellite phenomenon, hemolysis and serotype identification. The antimicrobial susceptibility showed that the isolated A. pleuropneumoniae resistance to penicillins, semisynthetic antibiotics, b-lactam antibiotics, polypeptide antibiotic, polyene antibiotic, coumarin antibiotic, amphenicols antibiotics, b-lactam/b-lactam inhibitor compounds, cephalosporins, aminoglycosides, tetracyclines, fluoroquinolones, macrolides, sulfa drugs, sugar peptide drugs, furan drugs, ethlhydrocupreine; medium sensitive to cephalothin (CF), cefizime (CEX), ciprofloxacin (CIP), norfloxacin (NOR), ofloxacin (OFL); high sensitive to polymyxin B (PB), fosfomycin (FOS), ceftizoxime (CTZ), cefmetazole (CMZ), cefaclor (CEC), cefoperazone/sulbactam (CFP/SU), cefetamet (CTM), cefotaxime (CTX), ceftazidime (CAZ), cefodizine (CDZ), cefurosimc sodium (CXM), cefotaxime/clayulanic acid (CTX/CA), ceftazidime/clavulanic acid (CAZ/CA), cefoxitin (FOX), cefepime (FEP), ceftriazone (CRO),aztreonam (AZT), minocycline. This result showed that A. pleuropneumoniae clinical strains had multi-drug resistance and resistance?characteristics, which means that these isolated bacteria was multiple drug resistant strains.

Keywords : Actinobacillus pleuropneumoniae; Isolation; Identification; Serotype; Antimicrobial resistance; antimicrobial susceptibiligy; Multidrug resistance.