Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Screening and Control Efficacy of Endophytic Bacteria CE3 from Castanea mollissima Blume

Li Linling1,3, Cheng Hua1,3, Hua Juan1,3, Yuan Honghui1,3, Xu Feng1 and Cheng Shuiyuan1,2*

1Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang, Hubei, China. 2College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China. 3College of life science, Huanggang Normal University, Huanggang, Hubei, China.

Received on 26 January 2015 and accepted on 07 March 2015

 

ABSTRACT

Castanea mollissima, as one of the important economic tree, is planted widely in China. In this work, a type of Bacillus cereus CE3 was isolated from healthy Luotian chestnut seed. From the colonization experiment of CE3 in chestnut seed, the antagonistic effect test and study of storage period on the seed induced resistance. The results showed that CE3 has antibiosis and induced resistance to the prevention mechanism of pathogenic?bacterium after picking chestnuts. It can also increase the POD and PPO dense enzyme activity and reduce the MDA content caused by chestnut postharvest pathogenic fungi. Besides, the colonization of dynamic test on the surface of chestnut showed that CE3 had strong affinity on the surface of Chestnut. In consequence, the mechanism for B.cereus preventing and controlling postharvest diseases of Chestnut included two sides: antibiotic effect and inducible resistance. By further speculating competing nutrition space might be also one of the mechanisms.

Keywords : Chestnut; Pathogenic fungi; Endophyte; Bacillus cereus; Biocontrol.