Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Antibiotic Sensitivity Test of E. coli Isolated from Drinking Water from Different Sources in and Around Ranchi, Jharkhand

Priscilla Kerketta1*, Ravuri Halley Gora2, Sushma Lalita Baxla2, Suruchi Kumari2 and Rajendra Yadava1

1Department of Veterinary Public Health and Epidemiology, 2Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Ranchi, Birsa Agricultural University, Kanke - 834006, India.

Received on 05 September 2014 and accepted on 25 October 2014

 

ABSTRACT

The present study was undertaken to isolate and determine the antibiotic sensitivity of E. coli, from drinking water samples collected from different sources in and around Ranchi, Jharkhand, India. The coliform count was done using most probable number (MPN). Isolation and identification of E. coli were done by morphological characteristics, enrichment media, selective media, and biochemical tests. Antimicrobial sensitivity testing by the disc diffusion method was conducted for 11 antibiotics: Amikacin, Amoxycillin, Ampicillin, Cephalothin, Chloramphenicol, Ciprofloxacin, Colistin, Cotrimoxazole, Enrofloxacin, Nalidixic acid and Oxytetracycline against isolated E.coli. The mean values of coliform MPN/100ml of water ranged from 0 to 1800+. Estimation of coliform in drinking water revealed that of 100 samples examined 47% samples had no coliform count, while in 6% samples the coliform count ranged between 1 to 10, in 7% samples the coliform count ranged between 11 to 20, in 19% the coliform count ranged between 21 to 100 and in 21% samples the coliform count was above 100 coliform per 100 ml of water. The isolates were highly sensitive to drug such as Amikacin (96.1%) and Colistin (96.1%) followed by Ciprofloxacin (92.3%), Chloramphenicol (88.4%) and Cotrimoxazole (84.6%). Intermediate sensitivity was shown for drugs such as Cephalolthin (34.6 %), Oxytetracycline (26.9 %) and Nalidixic acid (15.3 %). Maximum resistant was shown for drugs such as Enrofloxacin (73%), Oxytetracycline (65.3 %) and Cephalolthin (26.9 %). Although most strains of E. coli are not pathogenic, their presence is indicative of the possible presence of pathogenic organisms and the antibiotic sensitivity testing proved that the isolated E.coli are sensitive to many antibiotics and resistant to few of them.

Keywords : Antibiotic sensitivity Test, Coliform count, E.coli, Water.