Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Molecular Identification of Invasion Diseases Agents of Acer negundo L. in South Kazakhstan

Moldir Turaliyeva1*, Arystanbek Yeshibaev1, Khalima Sartayeva2, Aigul Uspabayeva1 and Gulmira Elibayeva1

1South Kazakhstan State University named after M. Auezov, Shymkent, Kazakhstan. 2South Kazakhstan State Pedagogical University, Shymkent, Kazakhstan.

Received on 10 January 2015 and accepted on 20 March 2015

 

ABSTRACT

The Acer negundo L. is one of native species of flora in Kazakhstan and forms a basis of dendroflora in all settlements. For last 10 years development of unknown diseases of Acer negundo L. has been registered. By the molecular-genetic analysis it is established, that agents of these diseases are microfungi of Fusarium solani and Fusarium oxysporum. Identity number of nucleotide sequences of their ITS regions with controller FJ914886.1 and EU625403.1 from the international bank (GeneBank) is 100, 0 and 99, 0 %, respectively. Currently, Bacillus thuringiensis 4ant bacteria have been isolated, which are capable of inhibiting the growth of the phytopathogenic fungus, the causative agents of trunk disease infecting Acer negundo L. The Bacillus thuringiensis 4ant strain?s antagonistic properties bespeaks its practical value as a useful agent in the biological struggle against diseases that cripple the dendroflora of the South of Kazakhstan.

Keywords : Acer negundo L., verticillium wilt, anthracnose, phytopathogen, Fusarium, micromycetes, antagonists, molecular-genetic analysis, Bacillus thuringiensis.