Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Characterization of Brucellaphage using RAPD and microsatellite repeat marker

Lata Jain1*, Mayank Rawat1, Vinay Kumar2 and Deepti Chachra3

1Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar - 243 122, India. 2Directorate of Medicinal and Aromatic Plant Research, Boriavi, Anand, Gujarat - 387310, India. 3Department of Veterinary Microbiology, College of Veterinary And Animal Sciences, Guru Angad Dev Veterinary and Animal Science University, Ludhiana (Punjab)-India.

Received on 20 December 2014 and accepted on 16 January 2015

 

ABSTRACT

Brucellosis is an important zoonotic infection of worldwide significance. In this study the recently isolated lytic brucellaphage 'FLd' against Brucella abortus was characterized using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Out of 18 RAPD primers used, 15 primers were able to generate distinct finger prints / amplicons ranged from two to twelve fragments. The RAPD finger prints produced were distinct and highly reproducible. A total of 18 microsatellite repeat (ISSR) markers used in the study had revealed that the phage genome consists of di-nucleotide repeats of AG, TG and CT and tri-nucleotide repeat of AGC and ACG. The RAPD and microsatellite fingerprinting has generated the basic information about the genomic composition of the Indian isolate of Brucellaphage which could be useful in the identification and discrimination of new phage and high resolution brucellaphage diversity studies.

Keywords : Brucellaphage, Random amplified polymorphic DNA, ISSR marker