Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Soil Bacterial Diversity Analysis of Cotton Field under Organic and Inorganic Management using DGGE

Malik Ahmed Pasha1, Sumangala Bhat1, P.U. Krishnaraj1

1Department of Biotechnology, University of Agricultural Sciences Dharwad, India.

Received on 25 February 2015 and accepted on 02 March 2015

 

ABSTRACT

Microbial diversity is one of the important microbial parameters that would reflect the fertility and health of soil. The effect of inorganic (conventional) farming on soil microbial structure and function is not very definite as yet. Organic farming is known to improve soil microbial diversity and leads to sustainability. Bacterial community structure of short term organic and inorganic farm soil was studied using culture independent approach and denaturing gradient gel electrophoresis. The species richness, diversity and the distribution of bacterial species improved in organic farming, but the change is subtle. We expect that this difference will be significant under long term application of organics.

Keywords : Organic farming, metagenomics, diversity, denaturing gradient gel electrophoresis.