Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Comparison of Fluoride Level and Bacterial Count in Tap Water, Reverse Osmosis Purified Water and Non-reverse Osmosis Purified Water

Sachin Naik1, Sanjeev Khanagar2, Darshan Devang Divakar3#, Abdulaziz Abdullah Al Kheraif3, Ravikumar Ramakrishnaiah3, Obaid Alshahrani4, Ali Alahmari and Salem Alsulami6

1Department of Public Health Dentistry, SJM Dental College and Hospital, Karnataka, India. 2Department of Public Health Dentistry, KVG Dental College and Hospital, Karnataka, India. 3Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia. 4Dentist, KSU, Alfarabi Colleges, 5Dentist, MOH, Alfarabi Colleges, 6Dentist, Alfarabi Colleges.

Received on 14 June 2014 and accepted on 06 November 2014



Water is one of the most important elements for all forms of life. With the general population concerned with polluted water, tendencies toward purchasing bottled water and water filtration systems are high which is the basic need for life and questions are being raised as to whether fluoride and bacterial content in public water supplies is affected by these filters. The objective of this study was to determine and compare fluoride level by spectrophtometric method and bacterial count by CFU (colony forming units) in tap water, reverse osmosis purified water and non-reverse osmosis purified water. Results of this study showed that the mean fluoride level was 0.06?0.04 in reverse osmosis purified water, 0.18?0.06 in non-reverse osmosis purified water, 0.20?0.06 in tap water. Mean bacterial count was 3.8?3.70 in reverse osmosis purified water, 8.66?6.63 in non- reverse osmosis purified water, and 21.6?5.31 in tap water. Considering the beneficial effects of fluoride on dental caries prevention, this paper highlights that when drinking water is subjected to water purification systems these system reduced fluoride content significantly below the optimal level along with reducing the bacterial count and playing a major role in initiation of dental caries.

Keywords : Water purifiers, Tap water, Reverse osmosis (RO), Colony forming units (CFU), Point of entry (POE), Point-of-use (POU), Parts per million (ppm).