Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Isolation and Phylogenetic Analysis of Some Lipase Producing Bacteria from a Saline Lake

Mojtaba Lorpour1, Aboozar Kazemi1, Ahmad Gholami1,2, Fatemeh Dabbagh1,2, Salman Abbaszadeh1, Azam Safari1 and Younes Ghasemi1,2*

1Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

Received on 03 May 2014 and accepted on 24 June 2014

 

ABSTRACT

Lipases are a member of enzymes which catalyze the breakdown of triglycerides to glycerol and free fatty acids. In this experiment we isolated lipase production capability of halophilic bacteria form Maharloo hyper saline lake located in south of Iran. In addition, phylogenetic analysis of isolates was done. The screening and isolation of lipase producing bacteria were done on selective media and enzyme activities were assayed according to the tetrimetric method. Isolated strains were identified based on 16S rDNA genes, using universal primers. Phylogenetic tree were constructed with the neighbor-joining method and aligned using MEGA software version 4.0. Thirteen lipase producing bacteria were screened and isolated. Characterization of these potential isolates by 16S rRNA gene analysis found them related to Bacillus and Staphylococcus genera. Phylogenetic tree displayed about 99-100% relationship between bacteria. Bacillus sp. BCCS A21 was found as the highest lipase producing strain with 16.5 U/mL of supernatant activity. All isolates were able to grow comfortably in the media containing 0-7% of salt. Bacillus sp. BCCS A21 with the high ability of lipase production on salty media could be applied in such industrial processes.

Keywords : Lipase, Maharloo, Staphylococcus, Bacillus, isolation.