Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Identification of Staphylococcus aureus and Coagulase-negative Staphylococcus (CoNS) as well as Detection of Methicillin Resistance and Panton-Valentine Leucocidin by Multiplex PCR

Neda Razavi Davoodi1, Seyed Davar Siadat1, Farzam Vaziri1, Jalil Vand Yousefi2, Naser Harzandi2, Ali Rafi2, Bahareh Rajaei1, Mehrangiz Zanganeh3, Alireza Japoni Nejad1, Shahin Najar Peerayeh4 and Ahmad Reza Bahrmand1

1Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran. 2Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran. 3Islamic Azad University Tehran Medical Branch, Tehran, Iran. 4Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Received on 03 August 2014 and accepted on 28 October 2014

 

ABSTRACT

Methicillin-resistant S. aureus (MRSA) is one of the most important pathogens that cause a wide range of hospital and community-acquired infections worldwide. In the present study, the Multiplex PCR test was employed to detect the genes 16S rRNA (Staphylococcus genus specific), femA (encoding a factor essential for methicillin resistance), mecA (encoding high-level methicillin resistant) and lukS gene (encoding Panton-Valentine leukocidin [PVL]). The results showed that all isolates harbored the 16S rRNA. Fifty-six (56 %) of these were determined as methicillin-resistant S. aureus, while 21 (70 %) were methicillin-resistant coagulase-negative staphylococci. 9% of S. aureus isolates harbored the lukS gene. This multiplex PCR assay represents a simple, rapid, reliable approach for the detection of methicillin-resistant staphylococci, evaluate the frequency of virulence factor in community- associated MRSA and discrimination of S. aureus from CoNS isolates.

Keywords : coagulase-negative Staphylococcus (CoNS), Panton-Valentine Leucocidin (PVL), Multiplex PCR, methicillin.