Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Phenotypic and Genotypic Characterization of Staphylococcus aureus of Mastitic Milk Origin from Cattle and Buffalo for some Virulence Properties

Rahul Yadav1*, Sandeep Kumar Sharma2, Jyotika Yadav3, Prerna Nathawat4 and Anil Kumar Kataria4

1Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Sciences, Bikaner, Rajasthan University of Veterinary and Animal science, Bikaner-334001 (Rajasthan), India. 2Department of Veterinary Microbiology and Biotechnology. Post graduate institute of veterinary education and research, Jaipur. Rajasthan University of Veterinary and Animal science, Bikaner-334001 (Rajasthan), India. 3 Veterinary graduate, College of Veterinary and Animal Sciences, Hisar, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar-125001 (Haryana), India. 4Center for studies on wildlife management and health, Rajasthan University of Veterinary and Animal science, Bikaner-334001 (Rajasthan), India. 5Department of Veterinary Microbiology and Biotechnology. College of Veterinary and Animal Sciences, Bikaner. Rajasthan University of Veterinary and Animal science, Bikaner-334001 (Rajasthan), India.

Received on 28 October 2014 and accepted on 07 January 2014

 

ABSTRACT

Mastitis is one of the most important diseases of dairy animals causing enormous financial loss and Staphylococcus aureus has been held the most common pathogen causing mastitis. The disease process is influenced by various virulence factors possessed by these organisms. In the present study 32 isolates from mastitic milk samples from cattle and buffalo were identified as S. aureus on the basis of their colony characteristics, biochemical tests and 23S rRNA ribotyping. All the isolates were subjected to different tests to detect production of virulence fators viz. coagulase, DNase, TNase and slime. In the present study production of coagulase, DNase, TNase and slime was shown by 100%, 93.7%, 100% and 96.87% isolates. The genotyping for capsular genes (cap5K and cap8K) revealed that 22 isolates (68.75%) were positive for cap5K and seven isolates (21.87%) were recorded positive for cap8K genes whereas three isolates (9.37%) were found non-typable for both cap5K and cap8K.

Keywords : Buffalo, cap5 and cap8 genes, Cattle, Coagulase, DNase, mastitis, ribotyping, Staphylococcus aureus, slime, TNase.