Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Plasmid Borne opd Gene in Myroides odoratimimus SKS05- GRD for Degradation of Dimethoate

T. Shanmuga Priya, A. Saranya, R. Nitya Meenakshi, M.S. Jebil, K.A. Nidhiya and Suganthi Ramasamy*

Department of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore, 641 014, India.

Received on 10 October 2014 and accepted on 10 December 2014

 

ABSTRACT

Myroides odoratimimus SKS05-GRD (Nucleotide sequence accession no. JQ178355) was able to utilize Dimethoate as phosphorus source and it can tolerate Dimethoate up to 6.25mg/ml. The ability of the organism to produce Phosphatase enzyme was screened by agar plate method and Phosphatase activity. The organism consists of an indigenous plasmid of size more than 21kb and effective plasmid curing was observed at higher concentration of Ethidium Bromide (300?g/ml and 500?g/ml). Phosphatase enzyme activity and Organophosphate (opd) gene was located on the indigenous plasmid in M. odoratimimus SKS05-GRD. HPLC analysis of the degradation assayed at intervals of 10days revealed a decrease in concentration of Dimethoate in the soil from 0.0312mg at the 10th day to 0.0239mg at the end of 40 days.

Keywords : Dimethoate, Myroides odoratimimus, Organophosphate, Phosphatase, opd gene.