Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Characterisation of Selected Trichoderma Isolates with Antifungal Activity*

Chidanand A. Rabinal1, Sumangala Bhat1* and P. U. Krishnaraj2

1Department of Biotechnology, Agriculture College Dharwad, UAS Dharwad - 580005, India 2Department of Agricultural Microbiology, College of Agriculture Vijayapur, UAS Dharwad 586101, India.

Received on 02 September 2014 and accepted on 25 November 2014

 

ABSTRACT

Sclerotium rolfsii is a polyphagous pathogen that infects and cause seed/collar rot in more than 500 crop species. The present study was taken up towards developing an eco-friendly, alternate to chemical fungicide. Isolates of Trichoderma such as T. virens, T. harzianum, T. hamatum, T. viride, and T. koningii were evaluated to identify potent isolate. In dual culture assay, among the ten analysed, Trichoderma isolates, T. virens IABT1002 significantly suppressed (100%) the growth of S. rolfsii followed by T. virens IABT1010 (95.47%). The production of hydrolytic enzymes such as chitinase and glucanase were high in T. virens IABT1002 (90.42 pmol/?g/min) and T. viride IABT1044 (596.9 pmol/?g/min) respectively. In inverted plate assay to assay effect of volatiles, T. harzianum IABT1041 showed 71.53% of radial growth inhibition of the S. rolfsii. The performance of all isolates were evaluated in green house experiment and T. koningii IABT1252 had significantly reduced the percent disease infestation from 100% infestation in S. rolfsii treated to 55.55% infestation in T. koningii IABT1252 treated seeds.

Keywords : Trichoderma, Sclerotium rolfsii, Chitinase, Glucanase, Colloidal chitin, Laminarian, N-acetyl-glucosamine.