Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Phenotypic and Genotypic Haemolysin Properties of Staphylococcus aureus Obtained from Milk of Cattle and Buffalo with Clinical Mastitis

Rahul Yadav1*, Sandeep Kumar Sharma2, Jyotika Yadav3, Taruna Bhati1 and Anil Kumar Kataria1

1Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Sciences, Bikaner. Rajasthan University of Veterinary and Animal Science, Bikaner-334001 (Rajasthan), India. 2Department of Veterinary Microbiology and Biotechnology, Post graduate institute of veterinary Education and Research, Jaipur. Rajasthan University of Veterinary and Animal science, Bikaner-334001 (Rajasthan), India. 3College of Veterinary and Animal Sciences, Hisar. Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar-125001 (Haryana), India.

Received on 06 April 2014 and accepted on 19 August 2014

 

ABSTRACT

The qualitative and quantitative phenotypic expression of haemolysins along with presence of genes encoding a- and b-haemolysin were determined in 32 Staphylococcus aureus isolates from milk of cattle and buffalo with clinical mastitis. Overall haemolytic reactions on sheep blood agar revealed five (15.62%) isolates to show complete haemolysis, 20 (62.50%) isolates to show partial haemolysis, four (12.50%) isolates to show both complete and partial and three (9.37%) did not show any haemolysis. All the 32 (100%) isolates from both cattle (16) and buffalo (16) produced a-toxin, the maximum titre of which was 1: 5120. Whereas beta-toxin was produced by 11 (68.75%) cattle isolates and by seven (43.75%) buffalo isolates with maximum titre of 1:1280 and 1:240, respectively. Delta toxin was detected to be produced by only five (15.62%) isolates, two from cattle and three from buffalo. The genotypic characterization revealed an overall hla gene prevalence in 96.8% isolates from both cattle and buffalo wherein a single amplicons of 534 bp was produced while hlb gene was amplified by 84.3% (13 cattle and 14 buffalo isolates) producing single amplicon of 833 bp.

Keywords : Staphylococcus aureus, Cattle, Buffalo, Mastitis, Haemolysis, hla and hlb gene.